CLART PNEUMOVIR-48 – CLART PNEUMOVIR- Extraction AND Purification-48

• DESCRIPTION OF THE DETECTION SYSTEM

CLART® PneumoVir allows detect the 19 most frequent types and subtypes of viruses causative of human respiratory infections, in the most common clinical samples:

Adenovirus; Bocavirus; Coronavirus; Enterovirus (Echovirus); Influenza virus A (H3N2, H1N1 y H1N1/2009, the latter causative of Influenza A (H1N1/2009); Influenza virus B; Influenza virus C; Metapneumovirus (subtypes A and B); Parainfluenza virus 1, 2, 3 y 4 (subtypes A and B); Rhinovirus; Respiratory syncytial virus type (RSV-A) and Respiratory syncytial virus type B (RSA-B).

Detection is based on our CLART® technology: End-point Multiplex RT-PCR (reverse transcriptase PCR) amplification, of a of a virus fragment of 120-330 bp, followed by visualization in low-density microarray.

Starting material for both formats is extracted DNA/ RNA from respiratory samples (See Section 6 below).

In order to avoid false negative results, each PCR tube includes an Internal control of amplification, which detection ensures the proper performance of the amplification process.

Basically, PCR amplified products labelled with biotin, hybridize with their specific complementary probes immobilised in well-defined areas of the microarray. Subsequent incubation steps take place thereon: first, with a streptavidin-peroxidase conjugate, and second, with an odianisidine substrate. A non-soluble product precipitate thereafter in regions of the microarray where specific hybridization between amplified products and their specific probes has taken place.

Thereafter, analysis and interpretation of results are automatically performed by GENOMICA’s reader (CAR® or CLINICAL ARRAY READER), running tailor-made software. autoclart® plus may alternatively be used.