• DESCRIPTION OF THE DETECTION SYSTEM

CLART® ENTHERPEX enables to detect infections of the 8 human herpes viruses HSV-1, HSV-2, VZV, CMV, EBV, HHV-6, HHV-7 and HHV-8, and of the 3 most relevant viruses of the Enterovirus family from a clinical point of view, Poliovirus, Echovirus y Coxsackievirus, without allowing distinguish between them.

As starting material, different types of human samples may be used: swabs, serum, plasma, cerebrospinal fluid and biopsy.

Detection is based on our CLART® technology: PCR amplification of a virus fragment of 106–328 bp, followed by visualization in low-density microarray. The chosen sequence for each virus is highly conserved, while at the same time being specific enough so as to distinguish each virus type by means of specific probes.

Basically, PCR amplified products labelled with biotin, hybridize with their specific complementary probes immobilised in well-defined areas of the microarray. Subsequent incubation steps take place thereon: first, with a streptavidin-peroxidase conjugate, and second, with an o-dianisidine substrate.

A non-soluble product precipitates thereafter in regions of the microarray where specific hybridization between amplified products and their specific probes has taken place.

Thereafter, analysis and interpretation of results are automatically performed by GENOMICA’s reader (CAR® or CLINICAL ARRAY READER), running tailor-made software. Autoclart® plus may alternatively be used.

1- ENTHERPEX Brochure
2- CLARTENTHERPEX user manual
3-1- Pre-PCRENTEN
3-2- Post-PCREntherpexEN