• DESCRIPTION OF THE DETECTION SYSTEM

       Pneumo CLART bacteria® enables to detect the presence of the main bacteria causative of human respiratory infections, in the following clinical samples: Sputum, nasopharyngeal lavage/exudate/aspirate, bronco-alveolar lavage (BAL) and bronchial suction.

      Bacteria detectable with Pneumo CLART bacteria® are:

      • Staphylococcus aureus 1, 2
      • Streptococcus pneumoniae 2
      • Haemophilus influenzae 2
      • Haemophilus spp 2
      • Moraxella catarrhalis 2
      • Mycoplasma pneumoniae
      • Bordetella pertussis
      • Bordetella parapertusis
      • Bordetella bronchiseptica
      • Bordetella holmesii
      • Bordetella spp. 3

      1-Including detection of the transposon mecA, responsible of appearance of methicillin resistance. MecA detection might be due to the presence in the sample of S. aureus, but also of any other Coagulase-Negative Staphylococci (CNS). However, the present kit only reports mecA positive in the presence of S. aureus.

      2-These microorganisms may be present in the commensal flora of the patient on a regular basis; therefore, a positive result provided by the kit for those bacteria, should be assessed taking into account the previously mentioned circumstance.

      3-The Bordetella spp. positive result will exclusively be reported in cases when the software does not allow distinguish between the above-mentioned Bordetella species.

      Detection is based on our CLART® technology: End-point Multiplex PCR amplification, of a fragment of each bacteria of between 150-550 bp, followed by visualization in low-density microarray.

      Basically, PCR amplified products labelled with biotin, hybridize with their specific complementary probes immobilised in well-defined areas of the microarray. Subsequent incubation steps take place thereon:

      first, with a streptavidin-peroxidase conjugate, and second, with an o-dianisidine substrate. A non-soluble product precipitates thereafter in regions of the microarray where specific hybridization between amplified products and their specific probes has taken place.

      Thereafter, analysis and interpretation of results are automatically performed by GENOMICA’s reader (CAR® or CLINICAL ARRAY READER), running tailor-made software. Autoclart® plus may alternatively be used.

1- PneumoClart Brochure
2- PneumoCLARTBacteria user manual